JRC GMO-Amplicons | EURL GMFF

Record ID:	EF660615.1
Database:	nt	Go to original record
Description:	Pseudotaxus chienii voucher LS20061204 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence; chloroplast.
Taxon:	Pseudotaxus chienii
Original size:	920 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	524	+	524 bp

Amplicon:

524 bp

1     cgaaatcggt agacgctacg gacttaatcg aattgagcct tggtatggag
51    acttactaag tgatagcttc caaatacagg gaaccctggg atattttgaa
101   tgggcaatcc tgagccaaat ccggattata ggaacaatag ttcccttttc
151   tagcaaaggg ataggtgcag agactcgacg gaagctattc taacgactca
201   atatcatttg aattggaacc aatattctat ctacagagtg cagtatgtta
251   tttaacactt gaaaagtgtg atgtatcatc aatttgttct gggcttacct
301   agtccaaaat aaaattgact ttaattcttt aattcttaag ttataaagta
351   atccaattac tagataactt atctataaag atagtttatt ccatttttga
401   atgattggac gagaataaag atagagtcca attctacatg ttaatgccaa
451   caacaatgca aattgcagta ggaggaaaat ccgttggttt tataaaccgt
501   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	524	505	2.36e-08

Match details