JRC GMO-Amplicons | EURL GMFF

Record ID:	EF660606.1
Database:	nt	Go to original record
Description:	Austrotaxus spicata voucher ABG20060714 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence; chloroplast.
Taxon:	Austrotaxus spicata
Original size:	955 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	521	+	521 bp

Amplicon:

521 bp

1     cgaaatcggt agacgctacg gacttaaccg aattgagcct tggtatggaa
51    acttaccaag tgatagcttc caaatacagg gaatcctggg atattttgaa
101   cgggcaatcc tgagccaaat ccaattttac taagtttttt tagtagtaaa
151   gggataggtg cagagactcg acggaagcta ttctaacgat tcaatatcat
201   ttgaatttga ctcaatactc tctctatcta gagagtgcct tatgtaatta
251   aacaatgagt gttgtttatc atcaacaact caactcaatt aaaacttgag
301   ttattctagg cttgcctagt ttcaaattcg aattgaagga attattcatt
351   ttaattctta tctagaaaga tagataattt atttcatttt tggaataaac
401   gttggacaag gataaagata gagtccaatt ctacatgtca atgccaacaa
451   caatgcaaat tgcagtagga ggaaaatccg ttggttttct agactgtgag
501   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.34e-08
primerB	100.0	20 bp	0	0	521	502	2.34e-08

Match details