JRC GMO-Amplicons | EURL GMFF

Record ID:	EF660604.1
Database:	nt	Go to original record
Description:	Taxus brevifolia voucher 11835 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence; chloroplast.
Taxon:	Taxus brevifolia
Original size:	887 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	523	+	523 bp

Amplicon:

523 bp

1     cgaaatcggt agacgctacg gacttaatag aattgagcct tggtatggaa
51    acttactaag tgatagcttc caaatacagg ggaaccctgg aatattttga
101   atgggcaatc ctgatccaaa tccgtattat aggaacaata attttataga
151   aaagggatag gtgcagagac tcaacgggag atattctaac gacttaatat
201   catttgaatt tgaaccaata tcaatattct atctacagag tgtagtatgt
251   tattgaaaac tttgaaagtg ttctgtatca tcgttaaaac ttgtttcaac
301   gattagaact tgagttgttc taggcttgcc tagcttaatg aatacttaat
351   taaagtaatt caattaagaa ataaatataa tttattccat ttttgaatta
401   ttggacgagg ataaagatag agtccaattc tacatgtcaa tgccaacaac
451   aacaatgcaa attgcagtag tcggaaaatc cgttggtttt ataaaccgtg
501   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.35e-08
primerB	100.0	20 bp	0	0	523	504	2.35e-08

Match details