JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828210.1
Database:	nt	Go to original record
Description:	Dasistoma macrophylla isolate a1 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Dasistoma macrophylla
Original size:	1663 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	725	1275	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgatt gcgctgatag aggaatgaaa cttcagaaag gataaggata
251   tacgtatcta ttgaatacta tatcaaaatc aaatgattga ttaatgatgg
301   cccaaatcta gatctgtatt tttgaatacg aaaagtggaa gaattggtgt
351   gaatttcttc cataaagaat attcattcat caaatcattc actccatagt
401   ccgatagatc ttttttaaaa gatctgatta atcggacgag aataaagata
451   gagtcccatt ctacatgtca ataccggcaa caatgaaatt tatagtaaaa
501   ggaaaatccg tcgactttta aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details