JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828209.1
Database:	nt	Go to original record
Description:	Brachystigma wrightii isolate 1 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Brachystigma wrightii
Original size:	1667 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	727	1278	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tgcgctgata gaggaatgaa acttcagaaa ggatgaaggg
251   taaacgtatc tattgaatac tatatcaaaa tcaaatgatt gattaatgat
301   ggcccaaatc tgtatctgta tttttgaata cgaaaagtgg aagaattggt
351   gtgaatttct tccataaaga atattcattc atcaaatcat tcactccata
401   gtccgataga tctttttaaa agatctgatt aatcggacga gaataaagat
451   agagtcccat tctacatgtc aataccggca acaatgaaat ttatagtaaa
501   aggaaaatcc gtcgactttt aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details