JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828192.1
Database:	nt	Go to original record
Description:	Agalinis setacea isolate 7MD tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis setacea
Original size:	1669 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	728	1278	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgatt gcgctgatag aggaatgaaa cttcagaaag gatgaaggat
251   aaacgtatct attgaataga tatcaaaatc aaatgattga ttaatgatgg
301   ccccaatctc tatctgtatt tttgaatacg aaaagtggaa gaattggtgt
351   gaatttcttc cataaagaat attcattcat caaatcattc actccatagt
401   ccgatagatc tttttaaaaa gatctgatta atcggacgag aataaagata
451   gagtcccatt ctacatgtca atactggcaa caatgaaatt tatagtaaaa
501   ggaaaatccg tcgactttta aaatcgtgag ggttcaagtc cctctatccc
551   c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details