JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828190.1
Database:	nt	Go to original record
Description:	Agalinis purpurea isolate 6SC tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis purpurea
Original size:	1618 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	709	1247	+	539 bp

Amplicon:

539 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctgttttc ttaaaacaaa agttcagaaa
151   acgaaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgatt gcgctgatag aggaatgaaa gaaaggataa acgtatctat
251   tgaatagata gcaaaatcaa atgattgatt aatgatggcc caaatctcta
301   tctgtatttt tgaatacgaa aagtggaaga attggtgtga atttcttcca
351   taaagaatat tcattcatca aatcattcac tccatagtcc gctagatctt
401   tttgcaaaga tctgattaat cggacgagaa taaagataga gtcccattct
451   acatgtcaat actggcaaca atgaaattta taggaaaagg aaaatccgtc
501   gacttttaaa atcgtgaggg ttcaagtccc tctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.42e-08
primerB	100.0	20 bp	0	0	539	520	2.42e-08

Match details