JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828186.1
Database:	nt	Go to original record
Description:	Agalinis pulchella isolate 4FL tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis pulchella
Original size:	1616 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	720	1265	+	546 bp

Amplicon:

546 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tgcgctgata gaggaatgaa acttcagaaa ggatgaagga
251   taaacgtatc tattgaatac tatatcaaaa tcaaatgatt gattaatgat
301   ggccccaatc tctatctgta tttttgaata cgaaaagtgg aagaattggt
351   gtgaatttct tccataaaga atattcattc atcaaatcat tcactccata
401   gtccgataga gctttgatct gattaatcgg acgagaataa cgatagagtc
451   ccattctaca tgtcaatacc ggcaacaatg aaatttatag taaaaggaaa
501   atccgtcgac ttgaaaaatc gtgagggttc aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	546	527	2.46e-08

Match details