JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828183.1
Database:	nt	Go to original record
Description:	Agalinis plukenetii isolate 2FL tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis plukenetii
Original size:	1663 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	731	1299	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgatt gcgctgatag aggaatgaaa cttcgataga ggaatgaaac
251   ttcagaaagg atgaaggcta aacgtatcta ttgactagat atcaaaatca
301   aatgattgat taatgatggc cccaatctct atctgtattt ttgaatacga
351   aagtggaaga attggtgtga atttcttcca taaagaatat tcattcatca
401   aatcattcac tccatagtcc gatagatctt tttaaacaga tctgattaat
451   cggacgagaa taaagataga gtcccattct acatgtcaat actggcaaca
501   atgaaattta tagtaaaagg aaaatccgtc gacttttaaa atcgtgaggg
551   ttcaagtccc tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details