JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828181.1
Database:	nt	Go to original record
Description:	Agalinis paupercula isolate 4MA tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis paupercula
Original size:	1628 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	709	1248	+	540 bp

Amplicon:

540 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tgcgctgata gaggaatgaa agaaaggata aacgtatcta
251   ttgaatagat agcaaaatca aatgattgat taatgatggc ccaaatctct
301   atctgtattt ttgaatacga aaagtggaag aattggtgtg aatttcttcc
351   ataaagaata ttcattcatc aaatcattca ctccatagtc cgctagatct
401   ttttgcaaag atctgattaa tcggacgaga ataaagatag agtcccattc
451   tacatgtcaa tactggcaac aatgaaattt ataggaaaag gaaaatccgt
501   cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	540	521	2.43e-08

Match details