JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828179.1
Database:	nt	Go to original record
Description:	Agalinis oligophylla isolate 5LAg tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis oligophylla
Original size:	1601 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	664	1216	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tcctctgata gaggaatgaa acttcagaaa ggatgaagga
251   taaacgtatc tattgaatac tatatcaaaa tcaaatgatt gattaatgat
301   ggcccaaatg tctatctgta tttttgaata cgaaaagtgg aagaattggt
351   gtgaatttct tccataaaga ctattcattc atcaaatcat tcactccatg
401   gtccgataga tcttttttaa aagatctgat taatcggacg agaataaaga
451   tagagtccca ttctacatgt caataccggc aacaatgaaa ttgatagtaa
501   aaggaaaatc cgtcgacttt tcaaatcgtg agggttcaag tccctctatc
551   ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details