JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828159.1
Database:	nt	Go to original record
Description:	Agalinis harperi isolate 14NC tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis harperi
Original size:	1634 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	703	1246	+	544 bp

Amplicon:

544 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgatt gcgctgatag aggaatgaaa cttcaggaag gataaacgta
251   tctattgaat agatagcaaa atcaaatgat tgattaatga tggccccaat
301   ctctatctgt atttttgaat acgaaaagtg gaagaattgg tgtgaatttc
351   ttccataaag aatattcatt catcaaatca ttcactccat agtccgatag
401   atctttttta aaagatctga ttaatcggac gagaataaag atagagtccc
451   attctacatg tcaataccgg caacaatgaa atttatagta aaaggaaaat
501   ccgtcgactt ttaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	544	525	2.45e-08

Match details