JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828158.1
Database:	nt	Go to original record
Description:	Agalinis harperi isolate 13FL tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis harperi
Original size:	1657 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	719	1268	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    cctactaagt gataactttc aaattcagag aaaccctgga attaagaaaa
101   aagggcaatc ctgagccaaa tcctgttttc ttaaaacaaa agttcagaaa
151   acgaaaaaag gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagttgattg cgctgataga ggaatgaaac ttcagaaagg atgaaggata
251   aacgtatcta ttgaatagat atcaaaatca aatgattgat taatgatggc
301   ccaaatctct atctgtattt ttgaatacga aaagtggaag aattggtgtg
351   aatttcttcc ataaagaata ttcattcatc aaatcattca ctccatagtc
401   cgatagatct tttttaaaag atttgattaa tcggacgaga ataaagatag
451   agtcccattc tacatgtcaa taccggcaac aatgaaattt atagtaaaag
501   gaaaatccgt cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details