JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828155.1
Database:	nt	Go to original record
Description:	Agalinis gattingeri isolate 1LA tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis gattingeri
Original size:	1652 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	718	1269	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaggggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tgcgctgata gaggaatgaa acttcagaaa ggatgaagga
251   taaacgtatc tattgaatag atatcaaaat caaatgattg attaatgatg
301   gcccaaatct ctatctgtat ttttgactac gaaaagtgga agaattggtg
351   tgaatttctt ccataaagaa tattcagtca tcaaatcatt cactccatag
401   tccgatagat cttttttaaa agatttgatg aatcggacga gaataaagat
451   agagtcccat tctacatgtc aataccggca acaatgaaat ttatagtaaa
501   aggaaaatcc gtcgactttt aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details