JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828149.1
Database:	nt	Go to original record
Description:	Agalinis edwardsiana isolate 1TX tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis edwardsiana
Original size:	1642 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	722	1275	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ttgcgcggat agaggaatga aacttcagaa aggatgaagg
251   ataaacgtat ctattgaata ctatatcaaa atcaaatgat tgattaatga
301   tggcccaaat ctctatctgt atttttgaat acgaaaagtg gaagaattgg
351   tgtgaatttc ttccataaag aatattcatt catcaaatca ttcactccat
401   agtccgatag atctttttta aaagatctga ttaatcggac gagaataaag
451   atagagtccc attctacatg tcaataccgg caacaatgaa atttatagta
501   aaaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details