JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828141.1
Database:	nt	Go to original record
Description:	Agalinis auriculata isolate 1 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis auriculata
Original size:	1607 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	728	1289	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tgcgctgata gaggaatgaa acttcagaaa ggatgaagga
251   taaacgtatc tattgaatac tatatcaaaa taaaatgatt gattaatgat
301   ggcccaaatc tgtatctgta tttttgaata cgaaaagtgg aagaattggt
351   gtgaatttct tccataaaga atattcattc atcaaatcat tcactccata
401   gtccgataga tcttttagat cttttttaaa agatctgatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aataccggca acaatgaaat
501   ttatagtaaa aggaaaatcc gtcgactttt aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details