JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828140.1
Database:	nt	Go to original record
Description:	Agalinis auriculata isolate 7IL tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis auriculata
Original size:	1621 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	709	1263	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tgcgctgata gaggaatgaa acttccgaaa ggatgaagga
251   taaacgtatc tattgaatac tatatcaaaa tcaaatgatt gattaatgat
301   ggcccaaatc tctatctgta tttttgaata cgaaaagtgg aagaattggt
351   gtgaatttct tccatcaaag aatattcatt catcaaatca ttcactccat
401   agtccgatag atcttttttt aaaagatctg attaatcgga cgagaataaa
451   gatagagtcc cattctacat gtcaataccg gcaacaatga aatttatagt
501   aaaaggaaaa tccgtcgact tttaaaatcg tgagggttca agtccctcta
551   tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details