JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828138.1
Database:	nt	Go to original record
Description:	Agalinis aphylla isolate 4AL tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis aphylla
Original size:	1628 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	711	1263	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttccgaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tgcgctgata gaggaatgaa acttcagaaa ggatgaagga
251   taaacgtatc tattgaatac tatatcaaaa tcaaatgatt gattaatgat
301   gggcccaatc tcgatctcta tttttgaata cgaaaagtgg aagaattggt
351   gtgcatttct tccataaaga atattcattc atcaaatcat tcactccata
401   gtccgatcga tctttttaaa aagatctgat taatcggacg agaataaaga
451   tagagtccca ttctacatgt caataccggc aacaatgaaa tttctagtaa
501   aaggaaaatc cgtcgacttt ttaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details