Match details

Record ID: EU070414.1
Database: nt Go to original record
Description: Glycine max putative truncated lectin (Le2) gene, promoter region and complete cds.
Taxon: Glycine max
Original size: 3929 bp

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Method: QT-TAX-GM-005 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of soybean lectin gene
Target:soybean lectin

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 1 1572 1646 + 75 bp

Amplicon: 75 bp
1     ccagcttcgc cgcttccttc aacttcactg ttcatgcgtc cgacatagca
51    aatctggcag atgggcttgc cttct 

View other 1 match with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 3.68e-09
primerB 95.2 21 bp 1 0 74 54 5.71e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.