JRC GMO-Amplicons | EURL GMFF

Record ID:	EF367911.1
Database:	nt	Go to original record
Description:	Lepidium perfoliatum voucher LEPE_13B tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Lepidium perfoliatum
Original size:	596 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	596	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctggtttatg cgaacaaacc ggagtttaga
151   aaacgagaaa aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt cactaccttg tgttgatatt gtgttgataa aggaatcctt
251   cgatcgaaac ttcaaatcaa aaaggatgaa ggagaaaaac ctatattgtc
301   taactatagg taacacaaaa gatctcaaaa atgacgacct gaatctcgat
351   ttctattttg tttataaaca aaatagaaat gttgtgaatc aattagaagt
401   ttaagaaaaa atcaactatt cattgaatca aatgattcac ttcatagtct
451   gatagatcct tggtggaact tattaatcgg acgagaataa agatagagtc
501   ccattctaca tgtcaatact gacaacaatg aaatttatag taagatgaaa
551   atccgttgac ttttaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details