JRC GMO-Amplicons | EURL GMFF

Record ID:	EF367852.1
Database:	nt	Go to original record
Description:	Lepidium montanum voucher LEMO_20B tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Lepidium montanum
Original size:	594 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	594	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctggtttacg cgaacaaacc ggagtttaga
151   aaacgagaaa aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt cactaccttg tgttgatatt gtgttgataa aggaatcctt
251   cgatcgaaac ttcaaatcaa aaaggatgaa ggagaaaaac ctatatttag
301   acaatatagg taacacaaaa gatctcaaaa atgacgacct gaatctcgat
351   ttttattttt ttataaacaa aataggaatg ttgtgaatca attcgaagtt
401   taagaaaaaa tcaactattc agtcatcaaa tgattcactt catagtctga
451   tagatccttg gtggaactta ttaatcggac gagaataaag atagagtccc
501   attttacatg tcaatactga caacaatgaa atttatagta agatgaaaat
551   ccgttgactt ttaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details