JRC GMO-Amplicons | EURL GMFF

Record ID:	FM179540.1
Database:	nt	Go to original record
Description:	Bixa orellana chloroplast tRNA-Leu gene (partial), trnL-trnF IGS and tRNA-Phe gene (partial), specimen voucher BONN:A.Worberg 40
Taxon:	Bixa orellana
Original size:	954 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	614	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaaa tcctgtttta caagaatgaa caaagattca
151   gaaagggaga ataaaaaaag gataggtgca gagactcaac ggaagctgtt
201   ctaacaaatg gggttgactg cctttgcgtt ggtaaagaaa tccttctatc
251   gaaattccgg atcggttcga atataggata aacctatata cataggtaat
301   gaaaaactat ctcaaaaaag acgagccgaa tctctatttt ttttttatta
351   tatgaaaaag aaaaagaatt gttgtgaatc gatttcaagt tgaagcaaag
401   aatcgaatag aatattcatt aatcaaatca ttcaccccat aatctgataa
451   atcttttgaa aaactgatta atcggacaag aataaagata gagtcccgtt
501   ctacatgtcg atatcaatat cgatcaatat taataccgac aacaatgaaa
551   tttatagtaa aaggaaaatc cgtcgactta aaaaancgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details