JRC GMO-Amplicons | EURL GMFF

Record ID:	EU145574.1
Database:	nt	Go to original record
Description:	Dibrachionostylus kaessneri isolate aG85 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Dibrachionostylus kaessneri
Original size:	1818 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	744	1326	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt cctaaaacaa acgttcagaa
151   agtgaaaaaa aggataggtg cagagactca acggaagctg ttctaacaaa
201   tggagttgga tgcgttagtc aacaagtctt tccaggaaaa attcctaaag
251   tatacgtatt taaggattaa gtgaaggata aacatatata catgcatccg
301   tagtgattaa atgagtaatg acagcccaaa tgaatctcta ttttttctat
351   gaaaaaaaaa agaattgttg ttaatagatt ccatgtttaa gaaagaatcg
401   aatattcatt gatcaagatg attcactcca tagtctgata gatcttttca
451   ggaaccgatt aatcagacga gaataaagat agagtcccat tttacatgtc
501   aatgccggca acaatgaaat ttatagttaa gaggaaaatc cgtcgacttt
551   taaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details