JRC GMO-Amplicons | EURL GMFF

Record ID:	EU145571.1
Database:	nt	Go to original record
Description:	Sherardia arvensis isolate aG64 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Sherardia arvensis
Original size:	1513 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	535	1117	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acctgctgag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttaaaaa
151   ggggataggt gcagagactc aatggaagct gttctaacaa atggagttgg
201   cctgtgttag tcgagaattc tttccatcaa aaattcagaa aatacaagta
251   ttaagtgaag gataaacata tatactatcc atacgcagtg aatagtatat
301   tataatgagt aatgacaacc ccaatgaatc tctacttttt catagaaaaa
351   atatagaatt gtagttgttg ttaaaatatt atacgtttaa gaaagaatcg
401   aatattcatt tatcaaatga ctcactccac agtctgatag atctttttac
451   gaactgatta attggacgag aataaagata gagtcccttt tctacatgtc
501   aatgccggca gcaatgaaat ttatagttaa taggaaaatc cgtcgacttt
551   taaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details