JRC GMO-Amplicons | EURL GMFF

Record ID:	EF571568.1
Database:	nt	Go to original record
Description:	Junellia aspera voucher R. G. Olmstead 2004-163 (WTU) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Mulguraea aspera
Original size:	1600 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	666	1230	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgacaaag ggggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgtgggt agaggaatct ttccatcgaa acttccgaaa
251   ggatgaagga taaacgtatc tattgaatac tctatcaaat gattaatgag
301   ggtccgaatc tgtatctgta ttttttgata tgaaaaaaaa tggaagaatt
351   ggtgtgaatt gattccacat tgaagaaaga atcgaatatt ccttgatcaa
401   atcattcact ccatagtccg ataaatcttt taaagaactg attaatcgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaacaatga
501   aatttattgt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details