JRC GMO-Amplicons | EURL GMFF

Record ID:	EF571566.1
Database:	nt	Go to original record
Description:	Junellia scoparia voucher R. G. Olmstead 2004-178 (WTU) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Mulguraea scoparia
Original size:	1611 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	677	1242	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgacaaag ggggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgtgggt agaggaatct ttccatcgaa acttccgaaa
251   ggatgaagga taaacgtatc tattgaatac tctatcaaat gattaatgag
301   ggtccgaatc tgtatctgta ttttttgata tgaaaaaaaa atggaagaat
351   tggtgtgaat tgattccaca ttgaagaaag aatcgaatat tccttgatca
401   aatcattcac tccatagtcc gataaatctt ttaaagaact gattaatcgg
451   acgagaataa agatagagtc ccattctaca tgtcaatacc ggcaacaatg
501   aaatttattg taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details