JRC GMO-Amplicons | EURL GMFF

Record ID:	EF571558.1
Database:	nt	Go to original record
Description:	Glandularia crithmifolia voucher R. G. Olmstead 2004-169 (WTU) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Junellia crithmifolia
Original size:	1588 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	667	1219	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctaaaaacaa aggttcagaa
151   aacgacaaaa ggggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctacgtgggt agaggaatct ttccatcgaa acttccgaaa
251   ggatgaagga taaacgtatc tattgaatac tctatcaaat gattaatgag
301   gtatatctgt attttttgat atgaaaaatg gaagaattgg tgtgaattga
351   ttccacattg aagaaagaat cgaatattcc ttgatcaaat cattcactcc
401   atagtccgat aaatctttta aagaactgat taatcggacg agaataaaga
451   tagagtccca ttctacatgt caataccggc aacaatgaaa tttattgtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details