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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: FM177582.1
Database: nt Go to original record
Description: Cloning vector pDEX00
Taxon: Cloning vector pDEX00
Original size: 10124 bp

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Method: QL-ELE-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998).
Target:T-nos

View other 15527 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 9865 10043 + 179 bp
Amplicon: 179 bp 
1     gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
51    acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
101   tgggtttttt gattagagtc ccgcaattat acatttaata cgcgatagaa
151   aacaaaatat agcgcgcaaa ctaggataa

View other 179 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 8.49e-09
primerB 100.0 20 bp 0 0 179 160 8.49e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.