JRC GMO-Amplicons | EURL GMFF

Record ID:	EF528487.1
Database:	nt	Go to original record
Description:	Cryptandra ericoides voucher D.E.Albrecht 3989 (MEL) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Cryptandra ericoides
Original size:	1004 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	603	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattacaaat
101   gggcaatcct gagccaaatc ctgttttctg aaaacaaaca agtattcaga
151   aagtgataat aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttggctgcga tgcgttagta aaggaatcct tccatcgaaa
251   ctccagaaag gatgaagaat aaacctatat atacgtgtac gtactgaaat
301   actatctcca aaccaaatga ttaatgacga cccgaatata tatatttttt
351   ttttatttat atgtttatat gaaaaacgaa agaattgttg tgaatcgatt
401   ccaagtaaaa aaaaatggaa tattcattga tcaaatcatt tactccatcg
451   taatctgata gatcttttga aaaatggatt aatcggacga gaataaagat
501   agagtcccat tctacatgtc aatatcgaca acaatgaaat ttatagtaag
551   aggaaaatcc gtcgacttta gaaatcgtga gggttcaagt ccctctatcc
601   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details