JRC GMO-Amplicons | EURL GMFF

Record ID:	MN564931.1
Database:	nt	Go to original record
Description:	Oxyria digyna chloroplast, complete genome.
Taxon:	Oxyria digyna
Original size:	160698 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48949	49550	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaacccggg aataaaaaaa
101   tgggcaatcc tgagccaact ccttctttcc atttccaaaa ggaagaataa
151   aaaaggatag gtgcagagac tcaacggaag ctgttctaac aaacaaatgg
201   acgatcttgc gttattataa aaattcttcc atcgaaactc taaaaaggat
251   gaagaataaa cctatatgca tatgcagacg tagtgaaata taaatactat
301   atattacata aaaaaattta atttttaaat attgataacg acccgcattt
351   tttttttatg actctgaaaa aaaggaagaa ttgttgcgaa gcgattccaa
401   gttgaagaaa gaatcgaata ctcgtttaat aaattaaagc atttattccc
451   cggtctgata gatcttttga agaaccgatc catcatatga gaatgaagat
501   agagtcccat tctacgtgtc aatcctgaca acaatgaaat ttatagtaag
551   aggaaaatcc gtcgacttta taaatcgtga gggttcaagt ccctctatcc
601   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details