JRC GMO-Amplicons | EURL GMFF

Record ID:	MN564930.1
Database:	nt	Go to original record
Description:	Rumex crispus chloroplast, complete genome.
Taxon:	Rumex crispus
Original size:	161292 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49000	49598	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtacggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   atgggcaatc ctgagccaac tcctcctttc caaaaggaag aataaaaaag
151   gataggtgca gagactcaat ggaagctgtt ctaacaaatg gagttgacga
201   tcttgcgtta gtagttatta taagaattct tccatcgaaa ctccaaaaaa
251   gatgaagaat aaacctatcc gcagacgtac ttaaatataa atactataac
301   tatatatata attattaaat taaaaaatat tgatgacgac ccgcattttt
351   ttatgactat gaaaagatgg aagaattgtt gtgaagcgat tccaagttga
401   agaaagaatc gactattcgt ttaataaatt aaagcattta ttccctgaag
451   tctgatagat cttttgaaga accgatccat cggatgagaa tgaagataga
501   gtcccattct acgtgtcaat cctgacaaca atgaaattta tagtaagagg
551   aaaatccgtc gactttataa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details