JRC GMO-Amplicons | EURL GMFF

Record ID:	MN564927.1
Database:	nt	Go to original record
Description:	Rheum pumilum chloroplast, complete genome.
Taxon:	Rheum pumilum
Original size:	161749 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49854	50464	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   atgggcaatc ctgagccaac tccttctttc caaaaggaag aataaaaaag
151   gataggtgca gagactcaat ggaagctgtt ctaacaaatg gagttgacga
201   tcttgcgtta ttataaaaat tcttccatcg aaactccaaa aaagatgaag
251   aataaaccta tacgcagacg tacttaaatc ttaaatataa atactatata
301   tataataact atatattata taaaaaaatt aaattaaaaa atattgatga
351   cgacccgcat ttttttatga ctatgaaaaa atggaagaat tgttgtgaag
401   cgattccaag ttgaagaaag agtcgaatat gcgtttaata aattaaagca
451   tttattcccc ggtctgagag atcttttgaa gaaccaatcc atcggatgag
501   aatgaagata gagtcccatt ctacgtgtca atcctgacaa caatgaaatt
551   tatagtaaga ggaaaatccg tcgactttat aaatcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details