JRC GMO-Amplicons | EURL GMFF

Record ID:	MN564926.1
Database:	nt	Go to original record
Description:	Rheum przewalskyi chloroplast, complete genome.
Taxon:	Rheum przewalskyi
Original size:	161740 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49425	50039	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   aatgggcaat cctgagccaa ctccttcttt ccaaaaggaa gaataaaaaa
151   ggataggtgc agagactcaa tggaagctgt tctaacaaat ggagttgacg
201   atcttgcgtt attataaaaa ttcttccatc gaaactccaa aaaagatgaa
251   gaataaacct atacgcagac gtactaaaat cttaaatata aatactatat
301   atataataac tatatattat atcaaaaaat taaaaattaa aaaatattga
351   tgacgacccg catttttttt atgactatga aaaaatggaa gaattgttgt
401   gaagcgattc caagttgaag aaagagtcga atatgcgttt aataaattaa
451   agcatttatt ccccggcctg agagatcttt tgaagaaccg atccatcgga
501   tgagaatgaa gatagagtcc cattctacgt gtcaatcctg acaacaatga
551   aatttatagt aagaggaaaa tccgtcgact ttataaatcg tgagggttca
601   agtccctcta tcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details