JRC GMO-Amplicons | EURL GMFF

Record ID:	MN564922.1
Database:	nt	Go to original record
Description:	Rheum acuminatum chloroplast, complete genome.
Taxon:	Rheum acuminatum
Original size:	161306 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49032	49635	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   atgggcaatc ctgagccaac tccttctttc caaaaggaag aataaaaaag
151   gataggtgca gagactcaat ggaagctgtt ctaacaaatg gagttgacga
201   tcttgcgtta ttataaaaat tcttccatcg aaactccaaa aaagatgaag
251   aataaaccta tacgcagacg tacttaaata taaatactat atataataac
301   tatatattat ataaaaaaaa tgaaattaaa aaatattgat gacgacccgc
351   atttttttta tgactatgaa aaaatggaag aattgttgtg aagcgattcc
401   aagttgaaga aagagtcgaa tatgcgttta ataaattaaa gcatttattc
451   cccggtctga gagatctttt gaagaaccga tccatcggat gagaatgaag
501   atagagtccc attctacgtg tcaatcctga caacaatgaa atttatagta
551   agaggaaaat ccgtcgactt tataaatcgt gagggttcaa gtccctctat
601   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details