JRC GMO-Amplicons | EURL GMFF

Record ID:	MT328397.1
Database:	nt	Go to original record
Description:	Platostoma chinense chloroplast, complete genome.
Taxon:	Platostoma chinense
Original size:	152547 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47014	47556	+	543 bp

Amplicon:

543 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaag
151   aaaaaaaagg ataggtgcag agactcaatg gaagctgttc taacaaatgg
201   gagttgactg cgctggtaga ggaatctttc catggaaact ttagaaagga
251   tgaaggttaa acgcatctat tgaatactat atcaaatggt taatgatggc
301   ccaaatcttt taatatgaaa aatggaaaaa ttggtgtgaa ttgattctac
351   gttgaagaaa aaatcgaata ttcatcaact cattcgctcc acagtccgat
401   agatctttta aagaactgat taatcggacg agaataaaga tagagtccca
451   ttctacatgt caatatcggc aacaatgaaa tttatagtaa gaggaaaatc
501   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	543	524	2.44e-08

Match details