JRC GMO-Amplicons | EURL GMFF

Record ID:	MT174154.1
Database:	nt	Go to original record
Description:	Dipteryx ferrea isolate DIMIPER20008 tRNA-Leu (trnL) gene, intron; chloroplast.
Taxon:	Dipteryx ferrea
Original size:	622 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	5	615	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctascaag tgagaacttt caaattcaga gaaacccagg aattaacaat
101   gggaaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcata
151   aagcgagagt aaagtaaaaa aggataggtg cagagactca atggaagttg
201   ttcgaacaaa tggagttgac aacatttctt ttcgcgttag gaaaggaatc
251   cttccatcga aattccagaa aggaaaggat caatgataaa tatatatata
301   tatacgtata tgtactgaaa tactatttca attgattaat gaagactcca
351   aaaatctata tttttgaatt gaatatttat atcacaaatg aaagatgtga
401   atcaattcca agttgaagaa agaatcgaat attcattgat caaatcattc
451   actccatcat agcctgatag atcttttgaa gaactgatta atcagatgag
501   aataaagata gagtcccatt ctacatgtta ataccgacaa caatgaaatt
551   tatagtaaaa ggaaaatccg tcgactttag aaatcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details