JRC GMO-Amplicons | EURL GMFF

Record ID:	MN723867.1
Database:	nt	Go to original record
Description:	Indofevillea khasiana voucher lpssy306 chloroplast, complete genome.
Taxon:	Indofevillea khasiana
Original size:	159238 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	51697	52299	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aggcttcgaa
151   aacgagaaaa aaaaaggggt aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttgactacgt tgcgttagta aaggaatgaa tccttccctc
251   cagaaaagat gaaagataaa cgtattacgt actgaaatac tatttgatta
301   atgataaccc gaatttctat tttttattat atttatataa aaaatgatat
351   ataaaaatga acgaattgtt atgaatcgat tccaatatct ccaagttgaa
401   aaaagaatcg aatattcatt gatcaaatca ttaactccat catacatagt
451   ctgatagatc ttttgaagaa ctgattaatc agatgcgaat agaataaaga
501   tagagtccca ttctacatgt caataccgac aaaaatgaaa tttatagtaa
551   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details