JRC GMO-Amplicons | EURL GMFF

Record ID:	MT261150.1
Database:	nt	Go to original record
Description:	Calochortus venustus chloroplast, complete genome.
Taxon:	Calochortus venustus
Original size:	155688 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46688	47268	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaacaaag tttttatttc
151   taaacctcga ataaaaaaga taggtgcaga gactcaatgg aagctgttct
201   aacgaatgga gttgattacg ttacgttgcg ttggtaactg gaatccttct
251   atcaaaatta cggggaggat gccctgtata cgtatatata catactgata
301   tagtaaacga ttgattaatc gcgacccgaa tccatcatat agatatgaaa
351   aattcagagt tattgtgaat ctattccaat cgaagttgaa ggaagaattg
401   actattcagt gatcaaatta ttcattccag agtctgatag atcttttgaa
451   aaatagatta ttcggatgag aataaagaga gagtcccatt ctacgtgtca
501   ataccgacaa caatgaaatt tatagtaaaa agaaaatccg tcgactttat
551   aagtcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details