JRC GMO-Amplicons | EURL GMFF

Record ID:	MT261171.1
Database:	nt	Go to original record
Description:	Streptopus ovalis chloroplast, complete genome.
Taxon:	Streptopus ovalis
Original size:	157359 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46824	47416	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttta gaaaacaagg gtttaatttc
151   taaaactaga ataaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg gagttgatta cgttacgttg cgttggtagc tggaatcctt
251   ctatcaaaat tacggagagg atgccctata tacgtatata cgtatatata
301   catactgaca tagtaaacga ttgattaata atcgcggccc gaatccatta
351   tatatatgaa aaatcttaga gttattgtga atctattcca atcgaagttg
401   aagaaagaat cgaatattca gtgatcaaat tattcattcc ggagtctgat
451   agatcttttt aaaaacagat tattcggacg agaataaaga gagagtccca
501   ttctacgtgt caataccgac aacaatgaaa tttatagtaa aaggaaaatc
551   cgtcgacttt ataagtcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details