JRC GMO-Amplicons | EURL GMFF

Record ID:	MT261173.1
Database:	nt	Go to original record
Description:	Tricyrtis macropoda chloroplast, complete genome.
Taxon:	Tricyrtis macropoda
Original size:	155453 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47567	48152	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaagta aattgagcct tagtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga taaaacaagg atttaatttc
151   tcaaactaga ataaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg aagtttatta cgttacgttg gtagctggaa tccttctatc
251   aaaattacgg agaggatgcc tgtatacgta tatatacata ctgacatagt
301   aaacaattga ttaatcgcgg cccaaatcca ttatatatat atatgaaaaa
351   attgagagtt attgtgaatc tcttccaatc aaaattgaag gaagaatcga
401   atattcatat tcagtgatca aattattcat tccagagtct gatagatctt
451   ttgaaaaacg gattattcga acgagaataa agagagagtc ccattttacg
501   tgtcaatacc gacaacaatg aaatttagag taaaaggaaa atccgtcgac
551   tttataagtc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details