JRC GMO-Amplicons | EURL GMFF

Record ID:	MT261153.1
Database:	nt	Go to original record
Description:	Clintonia udensis chloroplast, complete genome.
Taxon:	Clintonia udensis
Original size:	156214 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46869	47470	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc ccttttttga gaaaaagcgt tgatattatt
151   aaaaccacaa tcaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg gagttgatta tgttacgtta cactggtaac cggaatcctt
251   ctatcaaaat tacggagagg atgcctgccc tatatacgta tatatacata
301   ctgacatagt aaacgattga ttaatcgcgg cccgaatcga atccattata
351   tatatatatg aaaaatataa gagttattgt gaatctattc catattccaa
401   tcgaagttga aggaagaatc gaatattcag tgatcaaatt attcattccg
451   gagtctgata gatcttttga aaaacggatt attcggacga gaataaagag
501   agagtcccat tctacgtgtc aataccgaca acaatgaaat ttatagtaaa
551   aggaaaatcc gtcgacttta taagtcgtga gggttcaagt ccctctatcc
601   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details