JRC GMO-Amplicons | EURL GMFF

Record ID:	MN128389.1
Database:	nt	Go to original record
Description:	Scutellaria kingiana chloroplast, complete genome.
Taxon:	Scutellaria kingiana
Original size:	152395 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48085	48629	+	545 bp

Amplicon:

545 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaacccccg gaattaataa
101   aaatgggtga tcctgatcca aatcctgttt tctgaaaaca aaggctcaaa
151   aaacgaaaaa tgggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttgg ctgcgttgtt agaggaatcc tttccgcgga aacttcagaa
251   aggatgaatc tattgaatac tatatcaaat gattaatgat ggcccgaatc
301   tgtatttttg aatatgaaaa aacggaagaa tcggtgtgaa ttgattccat
351   attgaaaaaa atcgaatatt cattcatcaa atcattcact ccacggtcca
401   ataggtcttt taaagaacta attaattgga cgagaataaa gatagagtcc
451   cattctacat gtcaataccg gcaacaatga aatttatagt aagaggaaaa
501   tccgtcgact ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	545	526	2.45e-08

Match details