JRC GMO-Amplicons | EURL GMFF

Record ID:	MN128388.1
Database:	nt	Go to original record
Description:	Holmskioldia sanguinea chloroplast, complete genome.
Taxon:	Holmskioldia sanguinea
Original size:	153272 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48063	48619	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaaaccccg gaattaataa
101   aaatgggtga tcctgatcca aatcctgttt tctcaaaaca aaggttcaaa
151   aaacgaaaaa agggataggt gcagagactc gatggaagct gttctaacaa
201   acggagttgg ctgcgttggt agaggaatcc tttccacgga aacttcagaa
251   aggatgaagg ataaccgtat ctattgaata ctatatcaaa tgattaatga
301   tggcccgaat ctgtattttt taatatgaaa aatggaagaa ttggtgtgaa
351   ttgattccac attgaagaaa aaatcgaata ttcattcatc aaatcattca
401   ctccatagtc cgatagatct tttaaagaat tgattaatcg gacgagaata
451   aagatagagt cccattctac atgtcaataa tggcaacaat gaaatttata
501   gtaagaagaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details