JRC GMO-Amplicons | EURL GMFF

Record ID:	MN128386.1
Database:	nt	Go to original record
Description:	Scutellaria amoena chloroplast, complete genome.
Taxon:	Scutellaria amoena
Original size:	151833 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47535	48090	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaacccccg gaattaataa
101   aaatgggtga tcctgatcca aatcctgttt tctgaaaaca aaggctcaaa
151   aaacgaaaaa tgggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttgg ctgcgttgtt agaggaatcc tttccgcgga aacttcagaa
251   aggatgaagg ataaacgtat ctattgaata ctatatcaaa tgattaatga
301   tggcccgaat ccgtattttt gaatatgaaa aaacggaaga atcggtgtga
351   attgattcca cattgaaaaa aatcgaatat tcattcatca aatcattcac
401   tccacggtcc aataggtctt ttaaagaact aattaattgg acgagaataa
451   agatagagtc ccattctaca tgtcaatacc ggcaacaatg aaatttatag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details