JRC GMO-Amplicons | EURL GMFF

Record ID:	MN128383.1
Database:	nt	Go to original record
Description:	Scutellaria orthocalyx chloroplast, complete genome.
Taxon:	Scutellaria orthocalyx
Original size:	152071 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47762	48311	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaaaccccg gaattaataa
101   aaatgggtga tcctgatcca aatcctgttt tctgaaaaca aaggctcaaa
151   aaacgaaaaa tgggataggt gcagagactc gatggaagct gttctaacaa
201   atggagttag ctgcgttgtt agaggaatcc tttccgcgga aacttcagaa
251   aggataaacg tatctattga atactatatc aaatgattaa tgatggcccg
301   aatctgtatt tttgaatatg aaaaacggaa gaatcggtgt gaattgattc
351   cacattgaaa aaaaaatgga atattcattc atcaaatcat tcactccacg
401   gtccaatagg tcttttaaag aactaattaa tcggacgaga ataaagatag
451   agtcccattc tacatgtcaa taccggcaac aatgaaattt atagtaagag
501   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details