JRC GMO-Amplicons | EURL GMFF

Record ID:	MN128382.1
Database:	nt	Go to original record
Description:	Scutellaria przewalskii chloroplast, complete genome.
Taxon:	Scutellaria przewalskii
Original size:	151675 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47447	47995	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaaaccccg gaattaataa
101   aaatgggtga tcctgatcca aatcctgttt tctgaaaaca aaggctcaaa
151   aaacgaaaaa tgggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttgg ctgcgttgtt agaggaatcc tttccgcgga aacttcagaa
251   aggataaacg tatctattga atactatatc aaatgattaa tgatggcccg
301   aatccgtatt tttgaatatg aaaaacggaa gaatcggtgt gaattgattc
351   cacattgaaa aaaaatcgaa tattcattca tcaaatcatt cactccacgg
401   tccaataggt cttttaaaga actaattaat tggacgagaa taaagataga
451   gtcccattct acatgtcaat accggcaaca atgaaattta tagtaagagg
501   aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details