JRC GMO-Amplicons | EURL GMFF

Record ID:	MN128380.1
Database:	nt	Go to original record
Description:	Tinnea aethiopica chloroplast, complete genome.
Taxon:	Tinnea aethiopica
Original size:	152450 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47691	48244	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaaaccccg gaattaagaa
101   aaatgggtga tcctgatcca aatcctgttt tctgaaaaca aagactcaaa
151   aaacgaaaaa agggataggt gcagagactc gatggaagct gttctaacaa
201   atggagttgg ctgcgttgtt cgaagaatcc tttccgtgga aacttcagaa
251   aggatgaaga taaacgtatc tattgaatac tatatcaaat gattaatgat
301   ggcccgaatc tgtatttttg aatatgaaaa acggaagaat tggtgtgaat
351   tgattccaca ttgaaacaaa tcgaatattc attcatcaaa tcattcactc
401   cacagtccga tagatctttt aaagaactaa ttaatcggac gagaataaag
451   atagagtccc attctacatg tcaataccgg caacaatgaa atttatagta
501   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details