JRC GMO-Amplicons | EURL GMFF

Record ID:	MN128379.1
Database:	nt	Go to original record
Description:	Wenchengia alternifolia isolate HN chloroplast, complete genome.
Taxon:	Wenchengia alternifolia
Original size:	152843 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48157	48728	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgagaactt tcaaattcag agaaaccccg gaattaataa
101   aaatgggcga tcctgatcca aatcctattt tatcaaaata ttttatcaaa
151   acaaaggttc aaaaaacgaa aaaaaaagga taggtgcaga gactcaatgg
201   aagctgttct aacaaatgga gttgactgcg ttggtagagg aatcctttcc
251   gaggaaactt cagaaaggat gaaggataaa cgtatctatt gaatactata
301   tcaaatgatt aatgatggcc cgaatccgta ttttttaata taaaaaatgg
351   aagaattgat gtgaattgat tccacattga agaaaaaatc gaatattcat
401   tcatcaaatc attcactcca cagtccgata gatcttttaa aggactgatt
451   aattggacga gaataaagat agagtcccat tctacatgtc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details