JRC GMO-Amplicons | EURL GMFF

Record ID:	MN128378.1
Database:	nt	Go to original record
Description:	Wenchengia alternifolia isolate VN chloroplast, complete genome.
Taxon:	Wenchengia alternifolia
Original size:	152171 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47966	48538	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaaaccccg gaattaataa
101   aaatgggcga tcctgatcca aatcctattt tatcaaaaca ttttatcaaa
151   acaaaggttc aaaaaacgaa aaaaaaaagg ataggtgcag agactcaatg
201   gaagctgttc taacaaatgg agttgactgc gttggtagag gaatcctttc
251   cgaggaaact tcagaaagga tgaaggataa acgtatctat tgaatactat
301   atcaaatgat taatgatggc ccgaatccgt attttttaat ataaaaaatg
351   gaagaattga tgtgaattga ttccacatta aagaaaaaat cgaatattca
401   ttcatcaaat cattcactcc acagtccgat agatctttta aaggactgat
451   taattggacg agaataaaga tagagtccca ttctacatgt caataccggc
501   aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg
551   agggttcaag tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details