JRC GMO-Amplicons | EURL GMFF

Record ID:	MN814868.1
Database:	nt	Go to original record
Description:	Schnabelia oligophylla chloroplast, complete genome.
Taxon:	Schnabelia oligophylla
Original size:	156768 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	120716	121258	-	543 bp

Amplicon:

543 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcctttttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatctt tccacggaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact atatcaaatg attcatgatg
301   acccgaatct ctatttttta gaagaattgg tgtgaattga ttccacattg
351   aagaaaaaat cgaatattca ttcatcaaat cattcactcc acagtccgat
401   agatctttga aagaattgat taatcggacg agaataaaga tagagtcccg
451   ttctacatgt caataccggc aacaatgaaa tttatagtaa gaggaaaatc
501   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	543	524	2.44e-08

Match details